Fermentas gel extraction manual

Description. GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer. The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp. Let solidify 20min. to 1hr. depending on gel concentration and size. Adjust agarose and 1x TBE volumes to make various percentages of gels. To make a higher or lower % gel, simply modify the amount of agarose you add. 2. Place gel in rig with TBE buffer (use the same concentrstion of buffer that you used to make the gel above). Description. GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer. The kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column. The kit can be used to purify DNA fragments from 25 bp to 20 kb in size with recovery rates up to 95%.Brand: Thermo ScientificTrade.

agarose (%, w/v) gel electrophoresis in TAE buffer. And the correct products were digested with EcoRI and purified with a gel extraction kit (Fermentas) according to the instructions provided. The purified fragments were ligated into pPIC9 K vector (digested with EcoRI) using the DNA ligase. The recombinant plasmid was. as added by Taq polymerase was isolated by 2% agarose gel (Fermentas gel extraction kit). Individual aptamers were cloned using a TOPO TA kit (Life Technologies). Miniprep DNA (Fermentas) was prepared, and the presence of the expected aptamer insert was confirmed by EcoRI digestion and 2% agarose gel electrophoresis. The PCR amplification product was gel purified using the GeneJET gel extraction kit (Fermentas) and sent for sequencing by Imagif, Plateforme de Séquençage à Haut Débit by Illumina sequencing with an adapted RNA-seq protocol. Sequences were analysed with FastQC software (Babraham Bioinformatics).

GeneJET PCR Purification Kit is designed for PCR reaction clean-up. The kit can be used for gel extraction by following the protocol of GeneJET Gel Extraction Kit, but DNA yield may be compromised. For best performance of DNA gel extraction, we recommend to use GeneJET Gel Extraction Kit. For a 3-in-1 kit for purification of PCR products from. extraction conditions are used for DNA extraction and purification simultaneously. Agarose gel electrophoresis (More et al. ), Gel filtration resins including Sephadex G (Kuske et al. ) and commercial products such as DNA binding columns (Miller et al. ) are used for DNA purification after the extraction. Let solidify 20min. to 1hr. depending on gel concentration and size. Adjust agarose and 1x TBE volumes to make various percentages of gels. To make a higher or lower % gel, simply modify the amount of agarose you add. 2. Place gel in rig with TBE buffer (use the same concentrstion of buffer that you used to make the gel above).